Middle East respiratory syndrome coronavirus shows poor replication but significant induction of antiviral responses in human monocyte-derived macrophages and dendritic cells.
Identifieur interne : 000F84 ( Main/Exploration ); précédent : 000F83; suivant : 000F85Middle East respiratory syndrome coronavirus shows poor replication but significant induction of antiviral responses in human monocyte-derived macrophages and dendritic cells.
Auteurs : Janne Tynell [Finlande] ; Veera Westenius [Finlande] ; Esa Rönkkö [Finlande] ; Vincent J. Munster [États-Unis] ; Krister Melén [Finlande] ; Pamela Österlund [Finlande] ; Ilkka Julkunen [Finlande]Source :
- The Journal of general virology [ 1465-2099 ] ; 2016.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Adulte, Cellules dendritiques (immunologie), Cellules dendritiques (virologie), Chimiokine CXCL10 (métabolisme), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Humains, Immunité innée, Macrophages (immunologie), Macrophages (virologie), Protéines de résistance aux myxovirus (métabolisme), Protéines virales (analyse), Réplication virale, Virus du SRAS (immunologie), Virus du SRAS (physiologie).
- MESH :
- analyse : ARN viral, Protéines virales.
- immunologie : Cellules dendritiques, Coronavirus du syndrome respiratoire du Moyen-Orient, Macrophages, Virus du SRAS.
- métabolisme : Chimiokine CXCL10, Protéines de résistance aux myxovirus.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Virus du SRAS.
- virologie : Cellules dendritiques, Macrophages.
- Adulte, Humains, Immunité innée, Réplication virale.
English descriptors
- KwdEn :
- Adult, Chemokine CXCL10 (metabolism), Dendritic Cells (immunology), Dendritic Cells (virology), Humans, Immunity, Innate, Macrophages (immunology), Macrophages (virology), Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (physiology), Myxovirus Resistance Proteins (metabolism), RNA, Viral (analysis), SARS Virus (immunology), SARS Virus (physiology), Viral Proteins (analysis), Virus Replication.
- MESH :
- chemical , analysis : RNA, Viral, Viral Proteins.
- chemical , metabolism : Chemokine CXCL10, Myxovirus Resistance Proteins.
- immunology : Dendritic Cells, Macrophages, Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- physiology : Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- virology : Dendritic Cells, Macrophages.
- Adult, Humans, Immunity, Innate, Virus Replication.
Abstract
In this study we assessed the ability of Middle East respiratory syndrome coronavirus (MERS-CoV) to replicate and induce innate immunity in human monocyte-derived macrophages and dendritic cells (MDDCs), and compared it with severe acute respiratory syndrome coronavirus (SARS-CoV). Assessments of viral protein and RNA levels in infected cells showed that both viruses were impaired in their ability to replicate in these cells. Some induction of IFN-λ1, CXCL10 and MxA mRNAs in both macrophages and MDDCs was seen in response to MERS-CoV infection, but almost no such induction was observed in response to SARS-CoV infection. ELISA and Western blot assays showed clear production of CXCL10 and MxA in MERS-CoV-infected macrophages and MDDCs. Our data suggest that SARS-CoV and MERS-CoV replicate poorly in human macrophages and MDDCs, but MERS-CoV is nonetheless capable of inducing a readily detectable host innate immune response. Our results highlight a clear difference between the viruses in activating host innate immune responses in macrophages and MDDCs, which may contribute to the pathogenesis of infection.
DOI: 10.1099/jgv.0.000351
PubMed: 26602089
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">In this study we assessed the ability of Middle East respiratory syndrome coronavirus (MERS-CoV) to replicate and induce innate immunity in human monocyte-derived macrophages and dendritic cells (MDDCs), and compared it with severe acute respiratory syndrome coronavirus (SARS-CoV). Assessments of viral protein and RNA levels in infected cells showed that both viruses were impaired in their ability to replicate in these cells. Some induction of IFN-λ1, CXCL10 and MxA mRNAs in both macrophages and MDDCs was seen in response to MERS-CoV infection, but almost no such induction was observed in response to SARS-CoV infection. ELISA and Western blot assays showed clear production of CXCL10 and MxA in MERS-CoV-infected macrophages and MDDCs. Our data suggest that SARS-CoV and MERS-CoV replicate poorly in human macrophages and MDDCs, but MERS-CoV is nonetheless capable of inducing a readily detectable host innate immune response. Our results highlight a clear difference between the viruses in activating host innate immune responses in macrophages and MDDCs, which may contribute to the pathogenesis of infection. </div>
</front>
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